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Signalling
Part:BBa_K091151:Design
Designed by: Erin Feeney Group: iGEM08_Davidson-Missouri_Western (2008-07-18)
pLas' Testing Construct D
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1147
Illegal AgeI site found at 1344 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2548
Design Notes
pLas' Testing Construct D has the coding region for LuxR preceding the region for LasR, but the order of these regions is reversed in pLas' Testing Construct C
Source
The sources of the quorum sensing components are the modified lasI regulatory region in P. aeruginosa and the lux components from V. fisheri.